The aim was to evaluate a method for visualizing fs laser pulse induced microincisions inside crystalline lens tissue. Porcine lenses were modified ex vivo by fs laser pulses to create defined planes at which lens fibers separate. Lens fiber orientation and fs laser-induced micro-incisions were examined using a confocal laser scanning microscope. Micro-incision visualization revealed different cutting effects depending on fs laser pulse energy, ranging from altered tissue scattering properties with all fibers intact to definite fiber separation with a wide gap. CLSM permits visualization and analysis and thereby control of fs laser pulse induced microincisions inside crystalline lens tissue.