Metal nanoparticles have been generated in deionized water and acetone by femtosecond-pulsed laser ablation. The expansion velocity was measured in?process in dependence on pulse energy us-ing particle tracking image velocimetry. Particle size distributions of silver were determined at peri-odic time intervals during the generation process, reaching stabilization level around 50 nm after 5·105 pulses. Electrophoretic mobility of laser-generated metal nanoparticles was studied by laser scattering velocimetry and the electrophoretic deposition of charged nanoparticles was used to gen-erate nanoparticle-derived nanostructures on 3-dimensional microstructured surfaces.