Application of the two-photon polymerization (2PP) technique for the fabrication of submicron-size relief of radial binary diffractive optical elements (DOE’s) is studied. Binary DOE’s for the formation of special longitudinal intensity distribution (axial light segment) are realized. Interferometric investigations of the diffractive relief produced by the 2PP-technique and investigations of optical properties of the formed elements are presented. Results of computer simulations are in good agreement with the experimental observations.