For more than a decade living cells and biomaterials (typically hydrogels) have been printed with la-ser-assisted bioprinting. Often, a thin metal layer is applied as laser-absorbing material, called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propelsforward a coated biomaterial.Different lasers with laser wavelengths from 193 to 1064 nanometer have been used. As ametalDRL gold, silver, or titanium layers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these lasers parameters on bioprinting with a metal DRL, comparing different wavelengths and pulse durations – except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration(in the range of 8 to 200 ns)are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell viability are analyzed.