Cell transfection is oneof the most important techniques in biomedical sciences. It describes the delivery of genetic material into living cells. Usually, this is achieved by common techniques like liposomal delivery, electroporation or viral transduction. In our strategy, we add spherical gold nanoparticles to the cells for three hours. The gold nanoparticles sediment to the surface of the cells. After this incubation period, the molecules which should be delivered into the cells are added and the cells are irradiated by a weakly focused laser beam. The interactionof laser light and gold nano-particles leadsto a permeabilization of the cell membrane such that the molecules can diffuse into the cells. We successfully employed an 850 ps pulsed laser system at a wavelength of 532 nm and an 120 fs pulsed laser system at a wavelength of 796 nm for this approach. Wedemonstrated delivery of fluorophores, Morpholinos, and proteins into cells while maintaining high cell survival rates.Furthermore, by increasing the laser power, cell ablation is also possible for therapeutic approaches.