Enabling hyperspectral acquisition for scanning laser optical tomography
Proc. SPIE 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX
54-59
2022
Type: Zeitschriftenaufsatz (non-reviewed)
DOI: 10.1117/12.2608075
Abstract
Scanning Laser Optical Tomography (SLOT) is a three-dimensional imaging technique usable on a micro- to mesoscale.
The technique is equivalent to computer tomography, using a laser instead of x-rays. With this technology, a set of twodimensional
images is acquired at different angles and subsequently processed into volume information by reconstruction
algorithms. Different contrast mechanisms can be used, depending on the application. Up until now transmission,
scattering, fluorescence and second harmonic generation have been established for SLOT imaging.
All of these contrast mechanisms are coupled to a specific narrow bandwidth, which is determined before the acquisition
starts and is dependent on the setup and application. In order to collect true hyperspectral information, a spectrometer and
a broadband light source have been integrated. This way, the amount of information is increased with each measurable
wavelength, leading to various improvements of the SLOT technology. The entire transmission and absorption spectra of
three-dimensional samples can now be measured and reconstructed.
Here, we present the current state of development of the hyperspectral SLOT. This includes the technical construction of
the hardware setup and the development of the software integration. Many challenges need to be overcome when
implementing spectroscopy in a tomographic setup. Solutions to specific problems, such as decreased resolution and focal
shift, will be presented. Finally, we will show the first results of hyperspectral SLOT imaging.