3D volume cross-section images with Scanning Laser Optical Tomography (SLOT)
a & b) Xibalbanus tulumensis. Überlagerung dreier Kanäle (rot: Streuung bei 635 nm, grün: Autofluoreszenz bei 532 nm Anregung, blau: Autofluoreszenz bei 405 nm Anregung. Skalierungsbalken = 5 mm. a) Projektionsansicht b) Schnittansicht des rekonstruierten 3D-Datensatzes.

Scanning Laser Optical Tomography (SLOT) allows for both displaying in-depth resolution cross-sectional images of transparent segments ex vivo, and in vivo for small organisms. Scattering and fluorescence are used as contrast mechanisms. Even large-scale samples can be examined with SLOT.

 


With SLOT, transparent samples can be volumetrically visualized (3D) and reconstructed. The digital 3D volume images are generated from projection image stacks. Furthermore, SLOT images of cleared ex vivo samples, opaque surfaces and tissue-engineered samples (safety level S1) are possible. With SLOT, small sample organisms, such as D. melanogaster and C. elegans, can even be displayed in vivo.

Both fluorescence with linear excitation and scattering can be used as contrast mechanisms. Projected transmission images can be made with coherent (monochromatic) light, a subsequent reconstruction, however, is not  necessary. In addition, telecentric projection images can be made from a 360° view angle area.

Should the samples, which can have a diameter of up to 1.5 cm and 4 cm in length, not be transparent, it can first be evaluated if the samples can be cleared. If this is possible, the samples can be prepared accordingly.

 

 

This service is, among others, relevant for the following areas:

Imaging processes:

  • Scanning Laser Optical Tomography (SLOT)

 

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