Image of bacterial colonization (Bacillus cereus) of a dental implant using Scanning Laser Optical Tomography

ZMTHe3 cells (canine cells), which have been perforated using the interaction between laser radiation on the gold nano particle surface and the cell membrane. Successfully perforated cells show green fluorescence. The cells have been successfully perforated only in the areas which have been irradiated.

Work of the group focusses on:

• Design and development of Scanning Laser Optical Tomography (SLOT)
• Marker-free imaging using intrinsic contrast mechanisms
• Large-scale volumetric imaging of functionalized implants
• High throughput cell transfection using plasmon resonances on nanoparticles

Current scientific work and projects:

• Evaluation of different contrast mechanisms for Scanning Laser Optical Tomography - SLOT
  • Volumetric imaging using tissue optical (intrinsic) contrast mechanisms
• Transregio SFB 37 subproject A2 „Optoporations-conveyed-DNA-transfection using the femtosecond (fs) laser“ (DFG)
  • Generation of transfected recombined HMGB1 cells for tumor vaccination
• Transregio SFB 37 subproject Q1 „Optical multimodal in vitro imaging of proliferation and migration processes on implant surfaces“ (DFG)
  • 3-D microscopic imaging of implant surfaces
  • In vitro time lapse studies on the proliferation and migration of biofilms
  • Scanning Laser Optical Tomography, in vitro/ex vivo
• Laser-based cell and tissue manipulation in the cluster of excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy) (DFG)
  • In situ cross-linking bioartificial tissue construction for regenerative medicine
  • Laser-induced nanoparticle-based cell transfection